آمینو اسید آنالایزر
precision
syringe, driven by a stepper motor with a resolution of 17 steps per μl.
A programmable wash program will flush the entire injection system to
avoid cross contamination of the sample.
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Multistep Separation
Only
two or three buffer solutions have to be combined to form the best
optimized buffer profile at any part of the separation program. No more
compromises by the limitation to four or five buffer changes.
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Integrated Vacuum Degasser
avoids the interruption of the
buffer pump by air bubbles without the need of bubble traps with varying
volumes, causing changes in retention times of the different amino
acids. |
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Cooled Reagent Storage
All buffer solutions, as well as
the Ninhydrine reagent are stored under inert gas pressure in a
refrigerated cabinet to avoid oxidation and air contamination
independent of the ambient environment. |
Separation Column Oven
integrated solid state column oven
with fast heating and cooling capability with a temperature range from
+5°C to +85°C independent of the ambient temperature. Up to twelve
programmable temperature steps can be used during one separation
program. |
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Integrated Autosampler
with a capacity of 120 vials in a
cooled sample tray. Volumes from 1μl to 100 μl can be injected without
any loss of sample. The injected volume is adjusted by a high |
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Flow Diagram S
433 |
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for the amino acid detection at 440 nm and 570 nm wavelengths. Summing option for both channels, for
single channel integration of all amino acids
Safety Devices
control pump pressures, temperatures and leakages
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High-Temperature Reactor
with
a programmable temperature range from ambient to 185°C with a coiled
capillary for the color reaction of the amino acid-ninhydrine complex.
Automatic flushing of the reactor coil with a washing solution after
each run prevents the blockage of the capillary.
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Optional Application
- Post column derivatisation with OPA (needs an optional Fluorescence Detector)
-
Carbohydrate determination for reducing sugars with Cu-bicinchoninate
post-column derivatisation (except the separation column, no additional
extras are needed)
- Polyamine determination with Ninhydrine or OPA post-column derivatisation.
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Integrated Reagent Dosing Pump
for Ninhydrine delivery and flushing of the reaction coil after each run. Programmable flow rate from 0.01 to
2.0 ml/min.
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Complete Inert Design
All materials coming into
contact with the buffer solutions and reagents are made of inert
materials as PEEK, PTFE, PVDF etc. Therefore, there is no need for using
special non-corrosive buffer compositions or reagents.
Integrated Dual-Channel Photometer
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temperaturereagentorganizer, consisting of thesame Amino Acid Reaction
Module and QuaternaryGradient Pump as the S 433
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The modular system design of the S 433 enables the budget minded buyer to use several components asparts for a standard HPLC
system without furthercosts. Another solution is our low-cost Amino
AcidAnalyzer S 430 with manual injection valve and ambient
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xchangeable Sample Loop made of PEEK
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| Sample Racks For up to 120
Samples |
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| Exchangeable Dosing Syringe |
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Autosampler
only inert materials come in contact with buffer solutions & samples
(except sample needle)
exchangeable sample loop PEEK
fixed and variable volume injection mode
integrated sample cooling
up to 120 samples
also usable as standard HPLC
Autosampler
optionally with pre-column
derivatisation mode
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| Intuitive Software Control |
Features
Complete control of Gradient Program, Column & Reactor Temperature
Programmable Sample Sequence with individual run times, gradient and temperature profiles
Automatic usage control of Buffer & Reagent solutions
Emergency-Program (SOS) feature in case of pressure errors
Manual control of each single unit
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Controlling
the complex features of the S 433 is made easy and intuitive by the
latest software technology. All status parameters are displayed on one
screen. Further details like the gradient programsteps or column oven
temperatures can be displayed as separated tables. The use of an
intuitive screen layout helps new users to start working with this
software faster than ever before. |
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| High Precision & Reproducibility |
| change all
important parameters to fit thedesired application from protein
hydrolysates,physiological fluids to sugar analysis andbiogene amines. |
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The Amino
Acid Analyzer's innovative design leads to both, a high sensitivity and a
high degree of reproducibility. Every single instrument of the system
is optimized in its role to provide the best achievable results. The
flexible design
of each instrument allows the user to |
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Amount
|
Area
|
Level
|
|
10 %
|
1727.800
|
1
|
25 %
|
4265.700
|
2
|
50 %
|
8445.800
|
3
|
75 %
|
12675.400
|
4
|
100 %
|
17102.907
|
5
|
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Gradient System Accuracy
Flexibility
does not lead to inaccuracy. The use of theS 2100 Solvent Delivery
System leads to highly repro-ducible results while granting all the
flexiblity of aquaternary gradient pump. Neither the retention times,nor
the linearity of the injected amounts are negativelyinfluenced by the
use of gradient buffer delivery asthe following examples demonstrate: |
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RSD
|
Ø Retention Time
|
Amino Acid
|
0.011 %
|
min. |
9.793750
|
Aspartic Acid
|
0.011 %
|
min. |
13.143750
|
Serine
|
0.026 %
|
min. |
22.215625
|
Glycine
|
0.035 %
|
min. |
38.24375
|
Tyrosine
|
0.011 %
|
min. |
43.631250
|
Histidine
|
0.019 %
|
min. |
46.037500
|
Lysine
|
|
|
 |
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RSD
|
Ø Concentration
|
Amino Acid
|
1.342 %
|
μmol/1 |
98.09725
|
Aspartic Acid
|
1.320 %
|
μmol/1 |
98.109625
|
Serine
|
1.165 %
|
μmol/1 |
98.518125
|
Glycine
|
1.426 %
|
μmol/1 |
97.900000
|
Tyrosine
|
1.895 %
|
μmol/1 |
96.966000
|
Histidine
|
1.398 %
|
μmol/1 |
99.327000
|
Lysine
|
|
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High Sensitivity
100 pMol / each Amino Acid
(with baseline subtraction) |
 |
capabilities, a Ninhydrine pump, a 4-channel vacuum
degasser and a refrigerated reagent organizer with integrate dinert gas application system.
|
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The S 433
system includes an autosampler with cooled sample storage and partial
loop fill technique without sample loss, as well as a 2-plunger buffer
pump, a dual beam photometer, a column oven with active cooling |
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TECHNICAL SPECIFICATION |
Amino Acid Reaction Module
integrated 2-plunger reagent dosing pump with adjustable flow rate (0.01 to 2.0 ml/min
built-in dual filter photometer (440 and 570 nm) with
constant signal output and signal summary option
programmable signal offset
three different risetimes selectable
temperature controlled column
oven (1 to 99° C ±1° C )with active cooling capability
- temperature controlled post-column derivatisation reactor (up to 199° C ±1° C
automatic valve for coil flushing
display of the actual system pressure
safety features (e.g. leakage of reactor and column, high pressure
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Refrigerated Reagent Organizer S 7130
for storing all reagents, buffers and wash solutions
front side operated
special valves for applying inert gas for oxygen-free storage.
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Autosampler S 5200
for automatic injection of samples. All parts which come in contact with
liquids are chemically inert and biocompatible (PEEK or PTFE
sampling system operating in x, y, and z-axis
variable sample dosage without any sample loss
loop overfill mode
reproducibility less than 1 % upon injection of 10 μl variable volume
memory effect less than 0.1 % depending on the selected washing procedure
large graphical display and keyboard for easy control
injection volume programmable in 1 μl increments
temperature controlled sample compartment (5 - 60° C
programmable washing procedure with selectable volumes
programmable port for purging
programmable sample sequence
optional: pre-column derivatisation mode
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Additional Applications
Sugar analysis for reducing sugars
biogene amines
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 |
Manual Amino Acid Analyzer S 430
Also
available is a low-costvariant equipped with amanual injection
valveinstead of the autosamplerand a reagent organizerwithout cooling
option. Thiscost-effective version is wellsuited for laboratories
withsmall quantities of samples.
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Solvent Delivery System S 2100
Quaternary Pump for the reliable and reproducible mixing of the buffer
solutions. All parts coming into contact with the buffers are chemically
inert (PEEK or PTFE
dual plunger pump with special design for low pulsation
(less than 1 %)
flow range depending on installed pump head
(0.05 to 9.99 ml/min)
maximum pressure up to 350 bar 5200 PSI
battery buffered programs stored for the amino acid
determination (hydrolysates and physiological fluids
graphic display of gradient profile A, B, C, and D
programmable mixing cycle for the buffer solution
integrated 4-channel vacuum degasser
programmable flushing procedure
extended diagnostic features (e.g. high pressure control
low pressure control
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Order Information
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Description
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Catalog No
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Automatic Amino Acid Analyzer S 433
(without data system)
|
001 20 11
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Automatic Amino Acid Analyzer S 433-D
(with data system)
|
002 20 12
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Manual Amino Acid Analyzer S 430
(without data system)
|
003 20 11
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Manual Amino Acid Analyzer S 430-D
(with data system)
|
004 20 11
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other configurations on request *
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Analytical Specifications
Hydrolysate sample: < 60 min. runtime
Physiological sample: < 120 min. runtime
sensitivity: 10 pMol
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Options
optional Fluorescence detector |
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آمینو اسید آنالایزر
شرکت طب و فن ایلیا نماینده انحصاری و رسمی دز ایران
تجهیزات آزمایشگاهی پیشرفته
|
