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صفحه اصلی arrow گروه محصولات arrow گروه داروسازی arrow دستگاه های آنالیتیکال arrow آمینو اسید آنالایزر

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آمینو اسید آنالایزر

آمینو اسید آنالایزر

CHROMATOGRAPHY


Amino Acid Analyzer


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آمینواسید آنالایزر
                                                                     
Automatic Amino Acid Analyzer S 433
columns, combined with optimized ready-to-use buffer solutions and chemicals, creates the right
answer for any routine or research problem in amino acid determination. More than 30 years experience in developing and operating sophisticated amino acid analyzers results in unmatched performance.
            The innovative automatic Amino Acid Analyzer
S 433 combines the advantages of the classical ion exchange separation method with the modern technique of high performance liquid chromatography. The complete package of sophisticated instrumentation, a wide variety of prepacked and tested separation



pulsation-reduced solvent and Ninhydrine delivery through two independent completely
inert dual-piston pumps





amino acid Analyzer amino acid Analyzer

 

 

precision syringe, driven by a stepper motor with a resolution of 17 steps per μl. A programmable wash program will flush the entire injection system to avoid cross contamination of the sample.
            
Multistep Separation
Only two or three buffer solutions have to be combined to form the best optimized buffer profile at any part of the separation program. No more compromises by the limitation to four or five buffer changes.
Integrated Vacuum Degasser
avoids the interruption of the buffer pump by air bubbles without the need of bubble traps with varying volumes, causing changes in retention times of the different amino acids.

Cooled Reagent Storage
All buffer solutions, as well as the Ninhydrine reagent are stored under inert gas pressure in a refrigerated cabinet to avoid oxidation and air contamination independent of the ambient environment.
Separation Column Oven
integrated solid state column oven with fast heating and cooling capability with a temperature range from +5°C to +85°C independent of the ambient temperature. Up to twelve programmable temperature steps can be used during one separation program.

Integrated Autosampler
with a capacity of 120 vials in a cooled sample tray. Volumes from 1μl to 100 μl can be injected without any loss of sample. The injected volume is adjusted by a high

 

amino acid Analyzer

 

 

 

 

 

 

 

 

 

 

                                                                                                                                            Flow Diagram S 433
amino acid Analyzer
                       for the amino acid detection at 440 nm and 570 nm wavelengths. Summing option for both channels, for
single channel integration of all amino acids
                             
Safety Devices
control pump pressures, temperatures and leakages
        
High-Temperature Reactor
with a programmable temperature range from ambient to 185°C with a coiled capillary for the color reaction of the amino acid-ninhydrine complex. Automatic flushing of the reactor coil with a washing solution after each run prevents the blockage of the capillary.
Optional Application
- Post column derivatisation with OPA (needs an optional Fluorescence Detector)
- Carbohydrate determination for reducing sugars with Cu-bicinchoninate post-column derivatisation (except the separation column, no additional extras are needed)
- Polyamine determination with Ninhydrine or OPA post-column derivatisation.


Integrated Reagent Dosing Pump
for Ninhydrine delivery and flushing of the reaction coil after each run. Programmable flow rate from 0.01 to
2.0 ml/min.

      Complete Inert Design
All materials coming into contact with the buffer solutions and reagents are made of inert materials as PEEK, PTFE, PVDF etc. Therefore, there is no need for using special non-corrosive buffer compositions or   reagents.
Integrated Dual-Channel Photometer
                                         temperaturereagentorganizer, consisting of thesame Amino Acid Reaction Module and QuaternaryGradient Pump as the S 433  


The modular system design of the S 433 enables the budget minded buyer to use several components asparts for a  standard HPLC system without furthercosts. Another solution is our low-cost Amino AcidAnalyzer S 430 with manual injection valve and ambient

amino acid Analyzer
amino acid Analyzer
xchangeable Sample Loop made of PEEK
amino acid Analyzer
Sample Racks For up to 120  Samples 

amino acid Analyzer
Exchangeable Dosing Syringe
Autosampler

      only inert materials come in contact with buffer solutions & samples
(except sample needle)
 
exchangeable sample loop  PEEK
 
fixed and variable volume injection mode
 
integrated sample cooling
 
up to 120 samples
 
also usable as standard HPLC
Autosampler

 optionally with pre-column
derivatisation mode

amino acid Analyzer

 

Consumables

  ready made buffer solutions
(each lot tested individually)

 separation columns for a variety of
applications with long term stability

 ready made Ninhydrine solution

 a variety of standard solutions

 

amino acid Analyzer
Reagent Organizer

  inert gas (N2) supply with
adjustable pressure to prevent
buffer/reagent oxidation and
contamination

 integrated cooling
amino acid Analyzer
amino acid Analyzer
Amino Acid Reaction Module

  only inert materials come in contact with buffer solutions & samples

 integrated 2-plunger reagent dosing pump with a flow rate from 0.01 to 2.0 ml/min



 programmable reactor temperature

 integrated column oven with programmable temperature gradient
and leakage sensor

- automatic washing of reactor coil
with washing solution after the last
sample

 integrated dual-channel photometer
for the detection of amino acids at
440 and 570 nm

Quaternary Gradient Pump

  only inert materials come in contact with buffer solutions & samples

 flow rate of 0.05 to 10.0 ml/min

 integrated 4-channel vacuum
degasser

 more than 100 programmable gradient steps with a resolution of 0.1%

 two-plunger pump for smooth eluent
delivery

 also usable as HPLC gradient pump


Intuitive Software Control
Features

  Complete control of Gradient Program, Column & Reactor Temperature

 Programmable Sample Sequence with individual run times, gradient and temperature profiles

 Automatic usage control of Buffer & Reagent solutions

 Emergency-Program (SOS) feature in case of pressure errors

 Manual control of each single unit
Controlling the complex features of the S 433 is made easy and intuitive by the latest software technology. All status parameters are displayed on one screen. Further details like the gradient programsteps or column oven temperatures can be displayed as separated tables. The use of an intuitive screen layout helps new users to start working with this software faster than ever before.


amino acid Analyzer

amino acid Analyzer

 

High Precision & Reproducibility
change all important parameters to fit thedesired application from protein hydrolysates,physiological fluids to sugar analysis andbiogene amines.          The Amino Acid Analyzer's innovative design leads to both, a high sensitivity and a high degree of reproducibility. Every single instrument of the system is optimized in its role to provide the best achievable results. The flexible design
of each instrument allows the user to

 

 

Amount
Area
Level

10 %
1727.800
1
25 %
4265.700
2
50 %
8445.800
3
75 %
12675.400
4
100 %
17102.907
5

 


Gradient System Accuracy
Flexibility does not lead to inaccuracy. The use of theS 2100 Solvent Delivery System leads to highly repro-ducible results while granting all the flexiblity of aquaternary gradient pump. Neither the retention times,nor the linearity of the injected amounts are negativelyinfluenced by the use of gradient buffer delivery asthe following examples demonstrate:

 

 

RSD
Ø Retention Time
Amino Acid
0.011 %
min. 9.793750
Aspartic Acid
0.011 %
min. 13.143750 
Serine
0.026 %
min. 22.215625
Glycine
0.035 %
min. 38.24375
Tyrosine
0.011 %
min. 43.631250
Histidine
0.019 %
min. 46.037500
Lysine

 

 

 


amino acid Analyzer

 

 

RSD
Ø Concentration
Amino Acid
1.342 %
μmol/1 98.09725
Aspartic Acid
1.320 %
μmol/1 98.109625
Serine
1.165 %
μmol/1 98.518125
Glycine
1.426 %
μmol/1 97.900000
Tyrosine
1.895 %
μmol/1 96.966000
Histidine
1.398 %
μmol/1 99.327000
Lysine

 

 

 


High Sensitivity
100 pMol / each Amino Acid
(with baseline subtraction)
                                amino acid Analyzer               
capabilities, a Ninhydrine pump, a 4-channel vacuum
degasser and a refrigerated reagent organizer with integrate dinert gas application system.
              
The S 433 system includes an autosampler with cooled sample storage and partial loop fill technique without sample loss, as well as a 2-plunger buffer pump, a dual beam photometer, a column oven with active cooling


TECHNICAL SPECIFICATION                                                       
Amino Acid Reaction Module

  integrated 2-plunger reagent dosing pump with adjustable flow rate (0.01 to 2.0 ml/min

 built-in dual filter photometer (440 and 570 nm) with
constant signal output and signal summary option

 programmable signal offset

three different risetimes selectable

 temperature controlled column
oven  (1 to 99° C ±1° C )with active cooling capability

- temperature controlled post-column derivatisation reactor (up to 199° C ±1° C

automatic valve for coil flushing

 display of the actual system pressure

 safety features (e.g. leakage of reactor and column, high pressure
 
Refrigerated Reagent Organizer      S 7130

for storing all reagents, buffers and wash solutions

 front side operated

special valves for applying inert gas for oxygen-free storage.

Autosampler S 5200

for automatic injection of samples. All parts which come in contact with liquids are chemically inert and biocompatible (PEEK or PTFE

 sampling system operating in x, y, and z-axis

 variable sample dosage without any sample loss

loop overfill mode

 reproducibility less than 1 % upon injection of 10 μl variable volume

memory effect less than 0.1 % depending on the selected washing procedure

 large graphical display and keyboard for easy control

 injection volume programmable in 1 μl increments

 temperature controlled sample compartment (5 - 60° C

 programmable washing procedure with selectable volumes

 programmable port for purging

programmable sample sequence

 optional: pre-column derivatisation mode
Additional Applications

  Sugar analysis for reducing sugars

 biogene amines
 
amino acid Analyzer                        Manual Amino Acid Analyzer   S 430


Also available is a low-costvariant equipped with amanual injection valveinstead of the autosamplerand a reagent organizerwithout cooling option. Thiscost-effective version is wellsuited for laboratories withsmall quantities of samples.



      
       

 

Solvent Delivery System S 2100

Quaternary Pump for the reliable and reproducible mixing of the buffer solutions. All parts coming into contact with the buffers are chemically inert (PEEK or PTFE

dual plunger pump with special design for low pulsation
(less than 1 %)

 flow range depending on installed pump head
(0.05 to 9.99 ml/min)

 maximum pressure up to 350 bar 5200 PSI

 battery buffered programs stored for the amino acid
determination (hydrolysates and physiological fluids

 graphic display of gradient profile A, B, C, and D

 programmable mixing cycle for the buffer solution

integrated 4-channel vacuum degasser

programmable flushing procedure

 extended diagnostic features (e.g. high pressure control
low pressure control



Order Information
 
Description
Catalog No
Automatic Amino Acid Analyzer S 433
(without data system)
001 20 11
Automatic Amino Acid Analyzer S 433-D
(with data system)
002 20 12
Manual Amino Acid Analyzer S 430
(without data system)
003 20 11
Manual Amino Acid Analyzer S 430-D
(with data system)
004 20 11
 other configurations on request *

Analytical Specifications

  Hydrolysate sample: < 60 min. runtime

 Physiological sample: < 120 min. runtime

 sensitivity: 10 pMol

 
Options

  optional Fluorescence detector

amino acid Analyzer
amino acid Analyzer

 

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آمینو اسید آنالایزر

شرکت طب و فن ایلیا نماینده انحصاری و رسمی دز ایران

تجهیزات آزمایشگاهی پیشرفته

 

 

منوی اصلی

تماس سریع
آدرس دفتر
 تهران، خیابان دکترفاطمی
روبروی وزارت کشور
خیابان چهارم، پلاک 21، واحد 3

تلفن:  31 -88981429 -021
تلفکس:     88980309 -021
ایمیل:     tofico@tofico.ir

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